Coding

Part:BBa_K4880000

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-07-13)


PaFS

This gene encodes for the farnesene synthase derived from Picea abies and has been codon optimized in cyanobacteria. This sesquiterpene synthase converts farnesyl pyrophosphate to α-farnesene.

Farnesene

Farnesene is an organic chemical with formula of C15H24, and is a colorless, water soluble compound with market value of about 100 RMB per Kilogram. In nature, Farnesene is found in grapefruit, and is used as alarm pheromones by termines. In aromatherapy, it has anti-inflammatory anti-fungal and antimicrobial qualities, and is attributed to being able to calm and sedate one’s mind. They can also help to manage chronic diseases like Multiple Sclerosis, Alzheimer’s, and irritable bowel syndrome.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1059
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Blanc-Garin V, Chenebault C, Diaz-Santos E, Vincent M, Sassi JF, Cassier-Chauvat C, Chauvat F. Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes. Biotechnol Biofuels Bioprod. 2022 Oct 14;15(1):110.

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